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Article number

LN2271462

Product Description

MMP12 Rat CLIA Kit

Alternative names

MMP12, HME, ME, MME, Macrophage Metalloelastase, Macrophage Elastase

Size

96 Tests

Product Type

Sandwich

Target

MMP-12

Reactivity

Rat

Applications

ELISA

Application & Usage

Detection Range: 15.625 - 1000 pg/mL. Sensitivity: 9.375 pg/mL.

Sample Type

Serum, Plasma, Cell supernatant

Shipping Conditions

ICE

Background

This CLIA kit uses the Sandwich- CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat MMP-12. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat MMP-12 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat MMP-12 , biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat MMP-12. You can calculate the concentration of Rat MMP-12 in the samples by comparing the RLU value of the samples to the standard curve.

Extra information

Colormetric

SWISSPROT #

Q63341

UNSPSC number

41116158