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Article number

LN2040687

Product Description

PEK Human, Mouse, Rat UNLB Antibody

Alternative names

PERK, DKFZp781H1925, E2AK3_HUMAN, EC 2.7.11.1, Eif2ak3, Eukaryotic translation initiation factor 2 alpha kinase 3, Eukaryotic translation initiation factor 2-alpha kinase 3, Heme regulated EIF2 alpha kinase, HRI, HsPEK, Pancreatic eIF2 alpha kinase, Pancreatic eIF2-alpha kinase, PEK, PRKR like endoplasmic reticulum kinase, PRKR-like endoplasmic reticulum kinase, WRS

Size

200 µL

Product Type

Polyclonal

Target

PEK

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

UNLB

Isotype

IgG

Immunogen

Synthesized peptide derived from human PEK/PERK.

Concentration

1 mg/ml

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Applications

WB, ELISA

Molecular Weight

130 kDa

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Recommended Dilution

WB 1:500-2000, ELISA 1:10000-20000

Storage Conditions

Store at -20°C, and avoid repeat freeze-thaw cycles.

Shipping Conditions

RT

Extra information

Oligomerization of the N-terminal ER luminal domain by ER stress promotes PERK trans-autophosphorylation of the C-terminal cytoplasmic kinase domain at multiple residues including Thr-982 on the kinase activation loop . Autophosphorylated. Phosphorylated at Tyr-619 following endoplasmic reticulum stress, leading to activate its tyrosine-protein kinase activity. Dephosphorylated by PTPN1/TP1B, leading to inactivate its enzyme activity. N-glycosylated. ADP-ribosylated by PARP16 upon ER stress, which increases kinase activity.

ProteinID

Eukaryotic translation initiation factor 2-alpha kinase 3, PRKR-like endoplasmic reticulum kinase, Pancreatic eIF2-alpha kinase, HsPEK

UNSPSC number

12352203