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Article number

LN1405505

Product Description

DeFA1 Human ELISA Kit

Alternative names

Neutrophil Defensin 1, Defensin, alpha 1, HNP-1, HP-1, HP1, HP 1-56, Neutrophil Defensin 2, HNP-2, HP-2, HP2, DeFA1, DeF1, DeFA2, MRS, DeFA1B

Size

96wells/kit, with removable strips.

Product Type

ELISA Kit

Target

DEFA1

Reactivity

Human

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

E. coli, D39-C94

Applications

ELISA

Application & Usage

LabNed’s human DEFA1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for DEFA1 has been precoated onto 96-well plates. Standards(E.coli,D39-C94) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for DEFA1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human DEFA1 amount of sample captured in plate.

Sample Type

For quantitative detection of human DEFA1 in cell culture supernates, serum and plasma (heparin, EDTA).

Kit components

a monoclonal antibody from mouse, a biotinylated polyclonal antibody from goat

Storage Conditions

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Shipping Conditions

ICE

Background

Defensin is a human protein that is encoded by the DEFA1 gene. Defensins are a family of antimicrobial and cytotoxic peptides thought to be involved in host defense. They are abundant in the granules of neutrophils and also found in the epithelia of mucosal surfaces such as those of the intestine, respiratory tract, urinary tract, and vagina. The protein encoded by this gene, defensin, alpha 1, is found in the microbicidal granules of neutrophils and likely plays a role in phagocyte-mediated host defense. Members of the defensin family are highly similar in protein sequence and distinguished by a conserved cysteine motif. Several alpha defensin genes are clustered on chromosome 8. This gene differs from defensin, alpha 3 by only one amino acid. This gene and the gene encoding defensin, alpha 3 are both subject to copy number variation.

Extra information

Assay Range: 312pg/ml-20000pg/ml, Sensitivity: <10pg/ml

NCBI GENE ID #

1667

NCBI OFFICIAL SYMBOL

DEFA1

NCBI OFFICIAL FULL NAME

defensin alpha 1

SWISSPROT #

P59665

ProteinID

Neutrophil defensin 1

UNSPSC number

41116158