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Article number

LN1300534

Product Description

Goat Anti Rat IgM (Fc specific) UNLB Antibody

Size

1 mL

Product Type

Secondary Antibodies

Reactivity

Rat

Cross-reactivity

The antiSerum does not cross-react with any other component of the Rat Ig system. Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail, however in double radial immunodiffusion a reaction with Mouse has been observed.

Host

Goat

Source

Highly purified normal IgM isolated from pooled rat serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Conjugate

UNLB

Applications

IP

Physical State

Delipidated, heat inactivated, lyophilized, stable whole antiserum No preservative added. Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal goat serum. No foreign proteins added.

Application & Usage

Immunoprecipitation. In immunoelectrophoresis use 2 μl or equivalent against 120 μl antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 μl in a 3 mm diameter center well and 2 μl serum samples (neat and serially diluted) in 2 mm diameter peripheral wells. Precipitin titre 1:32 when tested against pooled normal rat serum in agar-block immunodiffusion titration.

Storage Conditions

The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C: prolonged storage at or below -20°C. Reconstitute the lyophilized antiserum by adding 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not refrozen, and preferably used the same day. Lyophilized at +4° C--at least 10 years. Reconstituted at or below -20° C--3-5 years. Reconstituted at +4° C--7 days

Shipping Conditions

RT

Background

The reactivity of the antiserum is restricted to the Fc part of the IgM molecule. In immunoelectrophoresis and radial immunodiffusion, using various antiserum concentrations against normal rat serum a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified IgM. No precipitation reaction is obtained with purified IgG, IgA, and IgG/Fab fragments. In precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgM in rat serum and other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of rat IgM from serum or plasma.

UNSPSC number

12352203