The specific activity was found to be >14,000ATU/mg.
Purity
Greater than 98.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Each mg of protein was lyophilized from a sterile solution containing 20mM PBS pH-7 and 2% mannitol
Usage
Solubility/Reconstitution Instructions
It is recommended to reconstitute the lyophilized Hirudin in sterile 18MΩ-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Shipping Conditions
RT
Background
Background
Recombinant Hirudin is derived from yeast and the polypeptide chain contains 65 amino acids and its Mw is 6979.5 Dalton which is identical to natural Hirudin except for the substitution of leucine for isoleucine at the N-terminal end of the molecule and the absence of a sulfate group on the tyrosine at position 63.The Recombinant Hirudin is purified by proprietary chromatographic techniques.