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Article number

LN0129928

Product Description

ITF1 Human Peptide

Alternative names

E2A, E47, ITF1, VDIR, TCF-3, bHLHb21, BHLHB21, E2A, Transcription factor E2-alpha, Class B basic helix-loop-helix protein 21

Size

0.1 mg

Product Type

Peptide

Target

ITF1

Reactivity

Human

Applications

Block

Buffer

ITF1 peptide is supplied as 100 μg of dried peptide. When the peptide is reconstituted in 200 μL water, the concentration will be 0.5 mg/mL.

Physical State

Lyophilized

Application & Usage

ITF1 Peptide is used for blocking the activity of ITF1 antibody (catalog number 45-782). The ITF1 antibody ships at 0.5 mg/mL and the ITF1 Peptide ships as a 100 ug pellet. When peptide is reconstituted in 200 uL water, the concentration will be 0.5 mg/mL, identical to that of the antibody. The best starting ratio would be 1:1 (which means molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volume of peptide and antibody at the required dilution and leave at ambient temperature for 20 minutes. Then incubate and develop in parallel two blots: one with the antibody and one with the antibody which has been pre-absorbed with the provided peptide.

Storage Conditions

Store at -20°C. When reconstituted minimize freezing and thawing.

Shipping Conditions

ICE

Extra information

ITF1 peptide corresponds to 14 amino acids of ITF1.

Protein GI #

27777636

Accession #

NP_003191.1

NCBI GENE ID #

6929

NCBI OFFICIAL SYMBOL

TCF3

NCBI OFFICIAL FULL NAME

transcription factor 3

NCBI ORGANISM

Homo sapiens

SWISSPROT #

P15923

UNSPSC number

12352202