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Article number

LN4113833

Product Description

SPA-Cys Rec. Protein

Alternative names

Immunoglobulin G-binding protein A, IgG-binding protein A, Staphylococcal protein A, SPA

Size

100 mg

Product Type

Recombinant proteins

Target

SPA-Cys

Source

E. coli

Peptide sequence

MNAAQHDEAQ QNAFYQVLNM PNLNADQRNG FIQSLKDDPS QSANVLGEAQ KLNDSQAPKA DAQQNNFNKD QQSAFYEILN MPNLNEAQRN GFIQSLKDDP SQSTNVLGEA KKLNESQAPK ADNNFNKEQQ NAFYEILNMP NLNEEQRNGF IQSLKDDPSQ SANLLSEAKK LNESQAPKAD NKFNKEQQNA FYEILHLPNL NEEQRNGFIQ SLKDDPSQSA NLLAEAKKLN DAQAPKADNK FNKEQQNAFY EILHLPNLTE EQRNGFIQSL KDDPSVSKEI LAEAKKLNDA QAPKEEDSLE HHHHHHC

Purity

Greater than 96.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.

Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

SPA protein was lyophilized with no additives

Solubility/Reconstitution Instructions

It is recommended to reconstitute the lyophilized SPA in sterile 18MΩ-cm H2O not less than 0.1mg/ml, which can then be further diluted to other aqueous solutions.

Shipping Conditions

RT

Background

SPA-Cys His Recombinant produced in E. coli is a single non-glycosylated polypeptide chain with 6×His-tag and a Cys on C-terminus. SPA-Cys His is comprised of 5 IgG-binding domains E-D-A-B-C aligned in series containing 307 amino acids and having a molecular mass of 34.8kDa containing little or no carbohydrate.Cell wall binding region, cell membrane binding region and albumin binding region were removed to ensure the highest specific IgG binding.

SWISSPROT #

P38507

UNSPSC number

12352202